Real-time Polymerase Chain Reaction:Article Title: The impact of adiposity on adipose tissue-resident lymphocyte activation in humans
Article Snippet: Samples were quantified (Qubit 2.0 fluorimeter; Life Technologies, Paisley, UK) and 2 μg reverse transcribed to cDNA using a High Capacity Reverse Transcription Kit (Applied Biosystems, Warrington, UK). .. Real-time PCR was performed using a StepOne (Applied Biosystems) with predesigned primers and probes obtained from Applied Biosystems for the measurement of macrophages (CD68: Hs02836816_g1), T lymphocyte populations and subsets (CD3G: Hs00962186_m1; CD4: Hs01058407_m1; CD8A: Hs00233520_m1; FOXP3: Hs01085834_m1; GATA3: Hs00231122_m1; and TBX21: Hs00203436_m1) and for expression of GLUT4 (Hs00168966_m1), IRS2 (Hs00275843_s1), HSL (Hs00193510_m1), leptin (Hs00174877_m1), adiponectin (Hs00605917_m1), MCP-1 (Hs00234140_m1), RANTES (Hs00982282_m1), IP-10 (Hs01124251_g1), IL-6 (Hs00985639_m1), IL-8 (Hs99999034_m1), IL-10 (Hs00961619_m1), IL-1Ra (Hs00893626_m1), TNF-α (Hs99999043_m1), IL-1β (Hs01555410_m1) and IL-18 (Hs00155517_m1). ..
Article Title: Calorie restriction-induced leptin reduction and T-lymphocyte activation in blood and adipose tissue in men with overweight and obesity.
Article Snippet: .. Real-time PCR was performed using a StepOneTM (Applied Biosystems; MA, USA) with predesigned primers and probes obtained from Applied Biosystems for measurement of LEPTIN (Hs00174877_m1), ADIPONECTIN (Hs00605917_m1), GLUT4 (Hs00168966_m1), IRS2 (Hs00275843_s1), HSL (Hs00193510_m1), LPL (Hs01012567_m1), PPARγ (Hs01115513_m1), MCP1 (Hs00234140_m1), IL6 (Hs00985639_m1), IL8 (Hs99999034_m1), IL1Rα (Hs00893626_m1), and IL18 (Hs00155517_m1) expression. ..
Article Title: Plasma CD93 concentration is a potential novel biomarker for coronary artery disease.
Article Snippet: The selection of tag SNPs was performed using the Tagger algorithm in HaploView 4.0. (MIT ⁄Harvard Broad Institute, Cambridge, MA, USA) SNPs rs2749817 and rs17682515 are located downstream of the CD93 3¢ UTR region, SNP rs2749812 is located in the 3¢ UTR region, SNP rs3746731 in exon 1 encodes a nonsynonymous P541S change, and SNP rs1884654 is located upstreamof theCD93promoter region (Figure S2). .. Genotypes were determined by allelic discrimination on real-time PCR with predesigned primers and probes from Applied Biosystems (design identification-numbers and call rates for the SNPs are provided in Table S1). ..
Expressing:Article Title: The impact of adiposity on adipose tissue-resident lymphocyte activation in humans
Article Snippet: Samples were quantified (Qubit 2.0 fluorimeter; Life Technologies, Paisley, UK) and 2 μg reverse transcribed to cDNA using a High Capacity Reverse Transcription Kit (Applied Biosystems, Warrington, UK). .. Real-time PCR was performed using a StepOne (Applied Biosystems) with predesigned primers and probes obtained from Applied Biosystems for the measurement of macrophages (CD68: Hs02836816_g1), T lymphocyte populations and subsets (CD3G: Hs00962186_m1; CD4: Hs01058407_m1; CD8A: Hs00233520_m1; FOXP3: Hs01085834_m1; GATA3: Hs00231122_m1; and TBX21: Hs00203436_m1) and for expression of GLUT4 (Hs00168966_m1), IRS2 (Hs00275843_s1), HSL (Hs00193510_m1), leptin (Hs00174877_m1), adiponectin (Hs00605917_m1), MCP-1 (Hs00234140_m1), RANTES (Hs00982282_m1), IP-10 (Hs01124251_g1), IL-6 (Hs00985639_m1), IL-8 (Hs99999034_m1), IL-10 (Hs00961619_m1), IL-1Ra (Hs00893626_m1), TNF-α (Hs99999043_m1), IL-1β (Hs01555410_m1) and IL-18 (Hs00155517_m1). ..
Article Title: Adipose Tissue Responses to Breaking Sitting in Men and Women with Central Adiposity
Article Snippet: Realtime PCR was performed using a StepOne (Applied Biosystems, Warrington, UK). .. Predesigned primers and probes were obtained from Applied Biosystems for the measurement of expression of interleukin 6 (IL-6) (Hs00985639_m1), adiponectin (Hs00605917_m1), leptin (Hs00174877_m1), interleukin 18 (IL-18) (Hs00155517_m1), tumour necrosis factor alpha (TNF-α) (Hs99999043_m1), monocyte chemoattractant protein-1 (MCP-1) (Hs00234140_m1), 5’ AMP-activated protein kinase (AMPK) (Hs01562315_m1 and Hs00178903_m1 combined), glucose transporter type 4 (GLUT4) (Hs00168966_m1), hormonesensitive lipase (HSL) (Hs00193510_m1), insulin receptor substrate 1 (IRS1) (Hs00178563_m1), insulin receptor substrate 2 (IRS2) (Hs00275843_s1), sterol regulatory element binding protein 1c (SREBP-1c) (Hs01088691_m1), pyruvate dehydrogenase kinase isozyme (PDK4) (Hs00176875_m1), peroxisome proliferator-activated receptor γ (PPARγ) (Hs01115513_m1), peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) (Hs01016719_m1), RAC-alpha serine/threonine-protein kinase (Akt1) (Hs00178289_m1), adipose triglyceride lipase (ATGL) (Hs00386101_m1), fatty acid translocase (FAT)/CD36 (Hs00169627_m1), forkhead box protein O1 (FOXO1) (Hs01054576_m1), hexokinase 2 (HK2) (Hs00606086_m1), phosphoinositide 3-kinase 85α (PI3K-85α) (Hs00933163_m1), fatty acid synthase (FAS) (Hs00188012_m1), peptidylpropyl isomerase A (PPIA) was used as an endogenous control (21). ..
Article Title: Calorie restriction-induced leptin reduction and T-lymphocyte activation in blood and adipose tissue in men with overweight and obesity.
Article Snippet: .. Real-time PCR was performed using a StepOneTM (Applied Biosystems; MA, USA) with predesigned primers and probes obtained from Applied Biosystems for measurement of LEPTIN (Hs00174877_m1), ADIPONECTIN (Hs00605917_m1), GLUT4 (Hs00168966_m1), IRS2 (Hs00275843_s1), HSL (Hs00193510_m1), LPL (Hs01012567_m1), PPARγ (Hs01115513_m1), MCP1 (Hs00234140_m1), IL6 (Hs00985639_m1), IL8 (Hs99999034_m1), IL1Rα (Hs00893626_m1), and IL18 (Hs00155517_m1) expression. ..
Article Title: SIRT 1 activator including syringaresinol
Article Snippet: Then, cDNA was synthesized from the extracted RNA (1 μg/μL) using a reverse transcription system (Promega). .. The expression pattern of the SIRT1 and GAPDH genes was monitored using the synthesized cDNA and predesigned primers and probes (Applied Biosystems; SIRT1, Hs01009006_m1; GAPDH, Hs99999905_m1). .. PCR reaction and analysis were carried out using the Rotor-Gene 3000 system (Corbett Research, Sydney, Australia).
Binding Assay:Article Title: Adipose Tissue Responses to Breaking Sitting in Men and Women with Central Adiposity
Article Snippet: Realtime PCR was performed using a StepOne (Applied Biosystems, Warrington, UK). .. Predesigned primers and probes were obtained from Applied Biosystems for the measurement of expression of interleukin 6 (IL-6) (Hs00985639_m1), adiponectin (Hs00605917_m1), leptin (Hs00174877_m1), interleukin 18 (IL-18) (Hs00155517_m1), tumour necrosis factor alpha (TNF-α) (Hs99999043_m1), monocyte chemoattractant protein-1 (MCP-1) (Hs00234140_m1), 5’ AMP-activated protein kinase (AMPK) (Hs01562315_m1 and Hs00178903_m1 combined), glucose transporter type 4 (GLUT4) (Hs00168966_m1), hormonesensitive lipase (HSL) (Hs00193510_m1), insulin receptor substrate 1 (IRS1) (Hs00178563_m1), insulin receptor substrate 2 (IRS2) (Hs00275843_s1), sterol regulatory element binding protein 1c (SREBP-1c) (Hs01088691_m1), pyruvate dehydrogenase kinase isozyme (PDK4) (Hs00176875_m1), peroxisome proliferator-activated receptor γ (PPARγ) (Hs01115513_m1), peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) (Hs01016719_m1), RAC-alpha serine/threonine-protein kinase (Akt1) (Hs00178289_m1), adipose triglyceride lipase (ATGL) (Hs00386101_m1), fatty acid translocase (FAT)/CD36 (Hs00169627_m1), forkhead box protein O1 (FOXO1) (Hs01054576_m1), hexokinase 2 (HK2) (Hs00606086_m1), phosphoinositide 3-kinase 85α (PI3K-85α) (Hs00933163_m1), fatty acid synthase (FAS) (Hs00188012_m1), peptidylpropyl isomerase A (PPIA) was used as an endogenous control (21). ..
Pyrolysis Gas Chromatography:Article Title: Adipose Tissue Responses to Breaking Sitting in Men and Women with Central Adiposity
Article Snippet: Realtime PCR was performed using a StepOne (Applied Biosystems, Warrington, UK). .. Predesigned primers and probes were obtained from Applied Biosystems for the measurement of expression of interleukin 6 (IL-6) (Hs00985639_m1), adiponectin (Hs00605917_m1), leptin (Hs00174877_m1), interleukin 18 (IL-18) (Hs00155517_m1), tumour necrosis factor alpha (TNF-α) (Hs99999043_m1), monocyte chemoattractant protein-1 (MCP-1) (Hs00234140_m1), 5’ AMP-activated protein kinase (AMPK) (Hs01562315_m1 and Hs00178903_m1 combined), glucose transporter type 4 (GLUT4) (Hs00168966_m1), hormonesensitive lipase (HSL) (Hs00193510_m1), insulin receptor substrate 1 (IRS1) (Hs00178563_m1), insulin receptor substrate 2 (IRS2) (Hs00275843_s1), sterol regulatory element binding protein 1c (SREBP-1c) (Hs01088691_m1), pyruvate dehydrogenase kinase isozyme (PDK4) (Hs00176875_m1), peroxisome proliferator-activated receptor γ (PPARγ) (Hs01115513_m1), peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) (Hs01016719_m1), RAC-alpha serine/threonine-protein kinase (Akt1) (Hs00178289_m1), adipose triglyceride lipase (ATGL) (Hs00386101_m1), fatty acid translocase (FAT)/CD36 (Hs00169627_m1), forkhead box protein O1 (FOXO1) (Hs01054576_m1), hexokinase 2 (HK2) (Hs00606086_m1), phosphoinositide 3-kinase 85α (PI3K-85α) (Hs00933163_m1), fatty acid synthase (FAS) (Hs00188012_m1), peptidylpropyl isomerase A (PPIA) was used as an endogenous control (21). ..
Control:Article Title: Adipose Tissue Responses to Breaking Sitting in Men and Women with Central Adiposity
Article Snippet: Realtime PCR was performed using a StepOne (Applied Biosystems, Warrington, UK). .. Predesigned primers and probes were obtained from Applied Biosystems for the measurement of expression of interleukin 6 (IL-6) (Hs00985639_m1), adiponectin (Hs00605917_m1), leptin (Hs00174877_m1), interleukin 18 (IL-18) (Hs00155517_m1), tumour necrosis factor alpha (TNF-α) (Hs99999043_m1), monocyte chemoattractant protein-1 (MCP-1) (Hs00234140_m1), 5’ AMP-activated protein kinase (AMPK) (Hs01562315_m1 and Hs00178903_m1 combined), glucose transporter type 4 (GLUT4) (Hs00168966_m1), hormonesensitive lipase (HSL) (Hs00193510_m1), insulin receptor substrate 1 (IRS1) (Hs00178563_m1), insulin receptor substrate 2 (IRS2) (Hs00275843_s1), sterol regulatory element binding protein 1c (SREBP-1c) (Hs01088691_m1), pyruvate dehydrogenase kinase isozyme (PDK4) (Hs00176875_m1), peroxisome proliferator-activated receptor γ (PPARγ) (Hs01115513_m1), peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) (Hs01016719_m1), RAC-alpha serine/threonine-protein kinase (Akt1) (Hs00178289_m1), adipose triglyceride lipase (ATGL) (Hs00386101_m1), fatty acid translocase (FAT)/CD36 (Hs00169627_m1), forkhead box protein O1 (FOXO1) (Hs01054576_m1), hexokinase 2 (HK2) (Hs00606086_m1), phosphoinositide 3-kinase 85α (PI3K-85α) (Hs00933163_m1), fatty acid synthase (FAS) (Hs00188012_m1), peptidylpropyl isomerase A (PPIA) was used as an endogenous control (21). ..
Synthesized:Article Title: SIRT 1 activator including syringaresinol
Article Snippet: Then, cDNA was synthesized from the extracted RNA (1 μg/μL) using a reverse transcription system (Promega). .. The expression pattern of the SIRT1 and GAPDH genes was monitored using the synthesized cDNA and predesigned primers and probes (Applied Biosystems; SIRT1, Hs01009006_m1; GAPDH, Hs99999905_m1). .. PCR reaction and analysis were carried out using the Rotor-Gene 3000 system (Corbett Research, Sydney, Australia).
Polymerase Chain Reaction:
|
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 